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KMID : 0379119990270060424
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Korean Journal of Mycology
1999 Volume.27 No. 6 p.424 ~ p.429
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Characteristics of Polysaccharide Isolated from the Fruit Body and Cultured Mycelia of Phellinus linteus IY001
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이준우/Lee, June Woo
백성진/방광웅/김용석/한만덕/하익수/Baek, Sung Jin/Bang, Kwang Woong/Kim, Yong Seok/Han, Man Deuk/Ha, Ick Su
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Abstract
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This study was conducted to investigate the characteristics of polysaccharides isolated from the fruit body and cultured mycelia of Phellinus linteus IY001. All fractions were extracted by hot water, followed by ethanol precipitation (F-THE and M-HE) or ultrafiltration (M-HU) (F-TH, F-THE; fruit body, M-HE, M-HU; cultured mycelia). Among these fractions, F-TH fraction was obtained at the highest yields of 6.83% and yield of F-THE was at the level 2.79%. The carbohydrates of these fractions was found to be a heteroglucan composed of glucose, galactose, mannose, fructose, ribose and xylose by analysis of gas chromatography. The total carbohydrate contents of M-HE and M-HU fractions were 99.2%, and 86.0% respectively. The glucose content of M-HE, M-HU and F-THE ranged from 54 to 84.8% of the total monosaccharide. Amino acid pattern showed that all fractions contained a large amount of aspartic acid, glycine, glutamic acid, alanine. Serine and threonine were found to be involved in the linkage, O-linked type. These fractions, except F-TH, contained polysaccharides with the molecular weights of 12 kD and showed the characteristics of IR absorption for β-glucosides at 890 ㎝^(-1).
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